Team

Dr. Qian is a Research Professor of Women’s Hospital, Zhejiang University School of Medicine. He possesses a multidisciplinary research background spanning clinical medicine, molecular cell biology, systems biology, and computational biology. His research focuses on the tumor microenvironment, mechanisms of chromosomal instability and carcinogenesis, biomarkers for early cancer screening and diagnosis, and immunotherapy, among other areas. Leveraging cutting-edge technologies such as single-cell and multi-omics sequencing, he has made significant contributions to advancing the scientific understanding of the tumor microenvironment and its therapeutic targeting across multiple cancer types. His findings have been published in leading academic journals, including Nature Medicine, Cell Research, Cancer Discovery and Molecular Cell, with total citations exceeding 4,700.

Please check Dr. Qian’s CV and Publications for more details.

Dr. Liu Qiang is dedicated to the development of a novel proximity labeling technology, PUP-IT, for studying protein-protein interactions in living cells. Utilizing PUP-IT, he discovered a new pathway regulating mitophagy in intracellular mitochondria (related work is currently under submission). Moving forward, his research focuses on two main areas. The first involves the comprehensive use of biochemical and cellular biology techniques to investigate changes in signaling pathways associated with the development and progression of tumors, particularly ovarian cancer, within living cells. The second area of focus is the development of new technologies based on PUP-IT and other proximity labeling methods to discover and document the impact of intercellular communication on tumor cell development, drug resistance, and metastasis.
Selected Publications:
Qiang Liu * “A proximity-tagging system to identify membrane protein-protein interactions”. Nature Methods 5, 715–722 (2018).(IF 30.82, Research Highlight: Deane, C. PUP up the volume. Nat Chem Biol 14, 903, 2018) (Faculty of 1000: Exceptional).

Rong Sang is a postdoctoral researcher at Qian’s Lab. Currently, his research direction involves investigating the classification and related mechanisms of ovarian cancer through multi-omics data. Before the current lab, his PhD research centers on the development of neural stem cells in Drosophila, undertaken under the guidance of Professor Yang Xiaohang at the Institute of Genetics, Zhejiang University. Contact: sangrong920316@zju.edu.cn.

Selected Publications:

1. Rong Sang et al., Mxc, a Drosophila homolog of mental retardation-associated gene NPAT, maintains neural stem cell fate. Cell Bioscience 31;12(1):78.(2022)

Duojiao Chen is a postdoctoral researcher at Qian’s Lab. Her research focuses on developing novel tumor classification strategies for gynecological tumors, particularly endometrial cancer, studying tumor microenvironments, and investigating tumor progression and development mechanisms using single-cell multi-omics technologies. She has been awarded fellowships from the International Postdoctoral Exchange Fellowship Program (Talent-Introduction Program) in 2022 and the 75th China Postdoctoral Science Foundation in 2024.

Selected Publications:

1. Duojiao Chen et al., Nonlinear relationship between chromatin accessibility and estradiol-regulated gene expression. Oncogene 40(7):1332-1346(2021)
2. Duojiao Chen et al., Cryopreservation Preserves Cell-Type Composition and Gene Expression Profiles in Bone Marrow Aspirates From Multiple Myeloma Patients. Frontiers in Genetics 21:12:663487(2021)

I’m Rushuang Yan. I obtained my master’s and Ph.D from Zhejiang University , where my research focus on pathogen intestinal decolonization, exploring strategies to eliminate resistant pathogens from the gut microbiome. In 2023, I joined Prof. Qian’s lab as a Postdoctoral Researcher, and started to focus on tumor microbiome and its role in cancer immunotherapy. My current work aims to engineer bacteria for novel cancer treatments, leveraging synthetic biology and microbiome science to enhance immune responses against tumors.

Selected Publications:

1. Rushuang Yan et al., Anticolonization of Carbapenem-Resistant Klebsiella pneumoniae by Lactobacillus plantarum LP1812 Through Accumulated Acetic Acid in Mice Intestinal Front Cell Infect Microbiol 2021, 15:11:804253
2. Rushuang Yan et al., A Sequence Type 23 Hypervirulent Klebsiella pneumoniae Strain Presenting Carbapenem Resistance by Acquiring an IncP1 blaKPC-2 Plasmid. Front Cell Infect Microbiol 2021, 1:11:641830

Junling Shi is a postdoctoral researcher at Qian’s Lab. She earned Ph.D. in Genetics from Zhejiang University under the supervision of Prof. Jun Ma and Prof. Feng He. They investigated the functional roles of tsRNAs in regulating Drosophila metamorphosis. In August 2024, she joined the Prof. Qian’s lab. Their current research focuses on unraveling how SLFN11 influence chemotherapy adaptation in ovarian cancer, aiming to identify novel therapeutic strategies.
Selected Publications:

1. Junling Shi et al., tRNA-derived small RNAs are embedded in the gene regulatory network instructing Drosophila metamorphosis. Genome research, 33(12), 2119–2132. (2023)

I joined the lab since 2022
Five things about me：
   1. R is the best language in the biological world.
   2. Where Cells Reside, There I Abid
   3. Central Dogma 2.0 : DNA → RNA → Protein → Coffee → Science
   4. Story, story, tell me a story！
   5. Always do the right things, do interesting things.

Selected Publication:

1. Wenhua Sang#, Yiduo Zhou#, et al., Receptor-interacting protein kinase 2 is an immunotherapy target in pancreatic cancer. Cancer Discovery. 14(2):326-347 (2024) (# co-fisrt author; IF 28.2)

I am a doctoral candidate in Surgery with a specialized focus on Hepatobiliary and Pancreatic Surgery. My primary research investigates the disease microenvironment of biliary dilatation through single-cell transcriptomic atlas construction. By combining clinical insights from hepatobiliary disorders with single-cell RNA sequencing and spatial transcriptomics, I aim to decode cellular crosstalk, stromal reprogramming, and immune dysregulation patterns in biliary dilatation progression. My work emphasizes translating genomic discoveries into clinical applications, such as identifying microenvironment-specific biomarkers for early diagnosis or tailored surgical interventions.

My name is Xinyu Ye. I graduated with a Bachelor’s degree in Clinical Medicine from Wenzhou Medical University. In 2023, I joined QLab as a direct-track Ph.D. student in Obstetrics and Gynecology. My recent research focuses on developing novel multimodal approaches with single-cell resolution to study cell-cell interactions in complex systems such as the tumor microenvironment. By directly labeling cell cell interactions, I aim to unravel the diversity of the tumor microenvironment mainly in ovarian cancer.

Yuping Yang is currently a doctoral student with an educational background that bridges mathematics and microbiology. She completed her undergraduate studies in Mathematics and Applied Mathematics at Hangzhou Normal University from 2016 to 2020. Subsequently, she has been pursuing a PhD in Microbiology at Zhejiang University since 2020. Her research focuses on multi-omics analysis of COVID-19 vaccine immunity, while she also shows a keen interest in single-cell algorithms, demonstrating a strong enthusiasm for exploring the intersection of mathematics and biology.

My name is Tian Chen. I completed both my undergraduate and master’s studies at Nanjing Medical University, specializing in Obstetrics and Gynecology. My primary research focus has been on immunotherapy for ovarian cancer. In September 2024, I joined this laboratory to continue my research in the field of ovarian cancer, with the goal of contributing to the development of more effective treatments through innovative scientific approaches.

Hi, I’m Yuhua Liu. Here’s a simple self-introduction ：

1. The master’s graduate who enrolled in 2023 specializes in the research of the occurrence, development of ovarian cancer and its tumor microenvironment.
   
2. Code in the morning and conduct experiments in the afternoon.
   
3. My goal：Make meaningful achievements, Make gorgeous figures！
   
4. My motto：Only you know how sweet your own dreams are.
   

I am Jinjin Zheng, currently serving as a Research Assistant in the Single-Cell Multi-Omics Laboratory. Since joining the team in July 2021, I have been responsible for the laboratory’s single-cell sequencing technologies and have accumulated three years of hands-on expertise in sample processing.My job includes making single - cell suspensions from tissues and blood, preparing libraries, and establishing single - cell RNA sequencing (RNA - seq), whole - genome sequencing, and immune repertoire sequencing (TCR - seq and BCR - seq).

My name is Dong Qingling, and I come from Shandong Province. I obtained my bachelor’s degree in Biology from Shandong Agricultural University and earned my master’s degree in Basic Medicine from Zhejiang University. I joined the lab in 2022, where I have been focusing on bioinformatics, particularly single-cell analysis. I have participated in several single-cell projects involving endometriosis, breast cancer, and subarachnoid hemorrhage (SAH), gaining valuable experience in data analysis and interpretation. In the future, I plan to pursue a Ph.D. to further deepen my expertise in this field.

I am Junfeng Fan, currently serving as a Research Assistant in the Single-Cell Multi-Omics Laboratory. Since joining the team in July 2024, I have been engaged in research focusing on tumor microenvironment immunoregulation mechanisms and development of early cancer diagnostic technologies. My current research focuses on: Liquid Biopsy Technology Innovation: Developing biomarker systems for early ovarian cancer diagnosis through plasma cell-free RNA high-throughput sequencing (cfRNA-seq).